This product detects a band of approximately 130kDa in human heart tissue cell lysates.
Immunohistology - Paraffin, Western Blotting
This product recognizes human Apaf1 (Apoptosis activating factor 1), a ubiquitously expressed cytoplasmic protein.
Apaf1 is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
IgG concentration 1.0mg/ml
Antiserum to human Apaf1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
0.02% Sodium Azide (NaN3)
Purified IgG prepared by Immunoaffinity chromatography