Target Name
APAF1
Target Species
Human
Species Detected
Mouse, Rat
Application Notes
This product detects a band of approximately 130kDa in human heart tissue cell lysates.
Specificity
This product recognizes human Apaf1 (Apoptosis activating factor 1), a ubiquitously expressed cytoplasmic protein.
Apaf1 is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Concentration
IgG concentration 1.0mg/ml
Preparation
Antiserum to human Apaf1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Purification
Purified IgG prepared by Immunoaffinity chromatography
