Additional Application Information
HCV NS3 Genotype-6 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Direct ELISA; Western blot;
HCV NS3 although stable at 4 °C for 1 week, should be stored below -18 °C.
Please prevent freeze thaw cycles.
20mM Tris-Hcl pH-8, 8M urea &10mM B-ME.
The E.coli derived 22kDa recombinant protein contains the HCV NS3 genotype 1b immunodominant regions, amino acids 1450-1643. The protein is fused with b-galactosidase (114 kDa) at N-terminus, pI 5.43.
HCV NS3 protein was purified by proprietary chromatographic technique.
HCV NS3 protein is >95% pure as determined by 10% PAGE (coomassie staining).