Antibody Target: NFkB p65
Alternative Name: RELA, NFKB3, MGC131774
Antibody Type: Monoclonal (Clone 2A12A7)
Isotype: Mouse IgG1
Immunogen: Synthetic peptide from human NFkB
Gene ID: 19697
Appearance: Colorless liquid
Concentration: 0.2 mg/ml
Formulation: 100 µg (200 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot, Immunoprecipitation, Immunohistochemistry
Application & Usage: Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. A431 cells can be used as a positive control.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines, and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.