RUNX3 293 Cell Lysate

RUNX3 293 Cell Lysate - 400440		Imprimer
Download Datasheet RUNX3 293 Cell Lysate Cell Lysate Unit 0.1 mg Cat. No. 400440 Description Antigen standard for runt-related transcription factor 3 (RUNX3), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. Datasheet Applications ELISA WB IP Format This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). Alternate Names isoform 1 is encoded by transcript variant 1; core-binding factor, runt domain, alpha subunit 3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C; oncogene AML-2; acute myeloid leukemia 2 protein; core-binding factor subunit alpha-3; SL3-3 enhancer factor 1 alp; RUNX3; AML2; CBFA3; FLJ34510; MGC16070; PEBP2aC Accession No. NP_001026850 Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. Storage Store at -80 °C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C. Product is guaranteed 6 months from the date of shipment. Images For research use only, not for diagnostic or therapeutic procedures.

CliniSciences - 183, avenue Georges Clémenceau - 92000 - Nanterre - France
Tel : +33 9 77 40 09 09 - Fax : +33 9 77 40 10 11 - info@clinisciences.com

RUNX3 293 Cell Lysate - 400440