AP1G1 293 Cell Lysate

AP1G1 293 Cell Lysate - 409699		Imprimer
Download Datasheet AP1G1 293 Cell Lysate Cell Lysate Unit 0.1 mg Cat. No. 409699 Description Antigen standard for adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. Datasheet Applications ELISA WB IP Format This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). Alternate Names isoform a is encoded by transcript variant 1; clathrin-associated/assembly/adaptor protein, large, gamma 1; gamma adaptin; golgi adaptor HA1/AP1 adaptin gamma subunit; clathrin assembly protein complex 1 gamma large chain; gamma1-adaptin; adaptor protei; AP1G1; ADTG; CLAPG1; MGC18255 Accession No. NP_001025178 Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. Storage Store at -80 °C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C. Product is guaranteed 6 months from the date of shipment. Images For research use only, not for diagnostic or therapeutic procedures.

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AP1G1 293 Cell Lysate - 409699