Alternate Names / Synonyms: Human Ubiquitin, Ubiquitin, h-Ubiquitin, rh-Ubiquitin, recombinant human Ubiquitin, recombinant Ubiquitin, Ubiquitin
Source: E. coli
Appearance: Lyophilized solid
Molecular Weight: 8.6 kDa
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: In deionized water (~50 mg/ml)
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Ubiquitin is a small, evolutionarily conserved eukaryotic protein that can be attached to a wide variety of intraCellular proteins including itself. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target Cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous Cellular processes. The recombinant Ubiquitin is expressed from E. coli and purified by proprietary chromatographic techniques.
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans.