|
|
|
| Our products |
|
|
 |
|
|
|
|
|
|
|
|
| Other information |
|
|
|
Human Larynx Squamous cell carcinoma, Stage : N / A, Grade : 1, Male, Age : 76, Tumor Tissue Lysate, Soluble Protein Fraction - C011-002T1 |
| Imprimer |
Please contact your local IMGENEX distributor for pricing on these products. For a list of distributors, click here or go to www.imgenex.com/distributors.php | | , Cat. No. |
| | Catalog Numbers: | | Cat No | Description | Units | | | | | -- |
| | Tissue Bundled Set contains the following tumor & normal adjacent lysates (below). Lysates may also be purchased individually. |
| | Extraction 1, Soluble Protein Fraction |
| | Extraction 2, Insoluble Protein Fraction |
| | | | Diagnosis: | | Grade: | | Stage: | | Sex: | | TNM: | | Quantity: | | Age: | | | | Concentration: | | | | The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides. | | | Storage: | Aliquot single use volumes to avoid repeated freeze/thaw cycles. From time of receipt, this product is stable for 12 months at –70°C. | | | Lysate Preparation: | Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature. | | | | Extraction 1: | PBS, pH 7.4 | 1 µg/ml Aprotinin | 1 mM NaF | | Modified RIPA Buffer: | 1 mM EDTA | 1 µg/ml Pepstatin-A | 0.1% SDS | | 0.25% Na deoxycholate | 1 µg/ml Leupeptin | 1 mM PMSF | | 1 mM Na3VO4 | | | | Extraction 2: | PBS, pH 7.4, 5.0 M Urea, 2.0 M Thiourea, 50mM DTT, 0.1% SDS | | | Usage: | These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use. Potential applications MAY include WB, immunoprecipitation, protein-protein interactions, ligand binding, ELISA. | | Note: For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required. | | Warranty: | The products sold hereunder are warranted only to conform to the quantity and contents stated on the label at the time of delivery to the customer. There are no warranties, expressed or implied, that extend beyond the description on the label of the product. IMGENEX’s sole liability is limited to either replacement of the products or refund of the purchase . IMGENEX is not liable for property damage, personal injury, or economic loss caused by the product. | | Precautions: | This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately. | | | PATHOLOGY REPORT | | Catalog Numbers: | | | Tissue: | | | Location: | | | Diagnosis: | | | Stage: | | | Grade: | | | Sex: | | | Age: | | |  | Developed in partnership with PROTEIN BIOTECHNOLOGIES |  | | 11175 Flintkote Ave., Ste. E San Diego, CA 92121. Toll Free: (888) 723-4363 Fax: (858) 642-0937 Email: info@imgenex.com | | |
