Target Name
TNF-alpha / TNF
Target Species
Horse
Application Notes
Specificity
This product is specific for equine tumour necrosis factor alpha (TNF alpha).
TNF alpha is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
This product recognizes both native and recombinant equine TNF alpha. No detectable cross-reactivity was observed with bovine, porcine, mouse or rat TNF alpha.
Storage/Stability
Store at +4 °C or -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Concentration
IgG concentration 1.0 mg/ml
Preparation
Antisera to equine TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Purification
Purified IgG prepared by affinity chromatography on Protein G
