Target Protein
Principal Names: RNF31 antibody, ZIBRA antibody, FLJ10111 antibody, FLJ23501 antibody, zinc in-between-ring-finger ubiquitin-associated domain antibody, ring finger protein 31 antibody, MGC19975 antibody, zinc in-between-RING-finger ubiquitin-associated domain protein antibody Official Symbol: RNF31 Accession Number(s): NP_060469.4 Human GeneID(s): 55072 Immunogen
Peptide with sequence C-EEVPLGQSIPRRRK, from the C Terminus of the protein sequence according to NP_060469.4.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20 °C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 120kDa band observed in lysates of cell line HepG2 (calculated MW of 120kDa according to NP_060469.4). Recommended concentration: 1-3 µg/ml.
Species Reactivity
Tested: Human
Expected from sequence similarity: Human, Dog, Pig