Target Protein
Principal Names: adenosine A2b receptor antibody, ADORA2B antibody, HGNC:264 antibody, ADORA2 antibody, netrin-1 receptor antibody Official Symbol: ADORA2B Accession Number(s): NP_000667.1 Human GeneID(s): 136 Immunogen
Peptide with sequence CQADVKSGNGQ, from the C Terminus of the protein sequence according to NP_000667.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20 °C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments in Human Colon, Lung and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1 µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules . Recommended concentration, 3 µg/ml.
Species Reactivity
Tested: Human
Expected from sequence similarity: Human 
