Target Protein Principal Names: ADORA2A antibody, adenosine A2a receptor antibody, HGNC:263 antibody, ADORA2 antibody, RDC8 antibody, hA2aR antibody, adenosine A2 receptor antibody, adenosine receptor subtype A2a antibody Official Symbol: ADORA2A Accession Number(s): NP_000666.2 Human GeneID(s): 135
Peptide with sequence HELKGVCPEPPGLDD, from the C Terminus of the protein sequence according to NP_000666.2.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20 °C. Minimize freezing and thawing.
Applications Tested Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: This antibody has been successfully used on lysates of primary Rhesus microglia in Van der Putten et al, PMID: 19494284.
Species Reactivity Tested: Rhesus
Expected from sequence similarity: Human
This antibody has been successfully used in the following paper:
van der Putten C, Zuiderwijk-Sick EA, van Straalen L, de Geus ED, Boven LA, Kondova I, IJzerman AP, Bajramovic JJ.
Differential expression of adenosine A3 receptors controls adenosine A2A receptor-mediated inhibition of TLR responses in microglia.
J Immunol. 2009 Jun 15;182(12):7603-12.