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Goat anti-MNSOD (aa119-130) - EB10972 |
| Imprimer |
| Code | Name | Applications | Availability | Size | Species reactivity | | Grade | | | EB10972 | Goat Anti-MNSOD (aa119-130) Antibody | Pep-ELISA, WB |  | 100 µg specific antibody in 200 µl | Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish | | EB10972 (0.1 µg/ml) staining of Mouse Spinal Cord lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Click here to download PDF datasheet. Target Protein Principal Names: SOD2 antibody, superoxide dismutase 2, mitochondrial antibody, RP1-56L9.2 antibody, IPOB antibody, MNSOD antibody, MVCD6 antibody, Mn superoxide dismutase antibody, indophenoloxidase B antibody, manganese-containing superoxide dismutase antibody, superoxide dismutase [Mn], mitochondrial antibody Official Symbol: SOD2 Accession Number(s): NP_000627.2; NP_001019637.1 Human GeneID(s): 6648 Non-Human GeneID(s): 20656 (mouse), 24787 (rat) Important Comments: NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1) . Reported variants represent identical protein: NP_000627.2; NP_001019636.1. Immunogen Peptide with sequence C-EAIKRDFGSFDK, from the internal region of the protein sequence according to NP_000627.2; NP_001019637.1. Please note the peptide is available for sale. Purification and Storage Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20 °C. Minimize freezing and thawing. Applications Tested Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 24kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Spinal Cord lysates (calculated MW of 24.8kDa according to Human NP_000627.2, and 24.6kDa according to Mouse NP_038699.2). Recommended concentration: 0.01-0.1 µg/ml. Species Reactivity Tested: Human, Mouse, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish
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