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Anti-Ethenoadenosine (Clone : 1G4) - HM5005 |
| Imprimer |
Ethenoadenosine, mAb 1G4 Catalog #: HM5005 Quantity: 100 µg Availability: In stock Description: Ethenoadenosine, mAb 1G4 The reaction of chloroacetaldehyde (CAA) with DNA results in the formation of ethenoadenosine also known as 1,N6-ethenodeoxy-adenosine or etheno-A. CAA is formed through metabolization of for example vinyl chloride, a well established carcinogen. In RNA, after in vitro activation, etheno-A and etheno-C are the principle products of RNA damage. Etheno derivate formation may be highest in single-stranded DNA-regions. The monoclonal antibody 1G4 reacts with both the ribose and deoxyribose form of the adduct. The antibody is not cross reactive with non-modified DNA or the normal nucleotides. Specifications | Catalog number | HM5005 | | Product type | Monoclonal antibodies | | Quantity | 100 µg | | Formulation | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin. | | Application | Flow cytometry, Frozen sections, Immuno assays, Immuno precipitation, Western blot | | Use | For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. | | Isotype | Mouse IgG2 | | References | 1. Young, T et al; Development of techniques to monitor for exposure to vinyl chloride: monoclonal antibodies to ethenoadenosine and ethenocytidine. Carcinogenesis 1988, 9: 589
2. Krebs, C et al; Flow cytometric and immunoblot assays for cell surface ADP-ribosylation using a monoclonal antibody specific for ethenoadenosine. Anal Biochem 2003, 314: 108 | | Storage and Stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. | | Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. | |
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