Mouse anti-Human p73 (Clone : 5B789) - IMG-260

Background
p73 was identified as a long-lost cousin of tumor suppressor protein, p53 (4). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (3). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (2). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein (1).

Antigen
This antibody was raised against full-length human p73. IMG-260 reacts with an epitope that is located on the C-terminal region of human p73 only. It does not cross react with mouse p73 or with p53.

Application Notes
1. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described (Sayan et al, 2008). The ability of a given antibody to recognize a p73 fragment will depend on whether or not the antibody epitope is contained in the fragment. As p73 research continues to evolve, researchers are encouraged to refer to the published literature using the p73 antibodies to gain additional information.

Genebank Info (Protein)
NP_005418

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Reference
1. Kaghad M, Bonnet H, Yang A, Creancier L, Biscan JC., et al. Cell 90: 809-819 (1997).
2. Levrero M, De Laurenzi V, Costanz0 A, Gong J, Wang JY, and Melino G. J Cell Sci 113 (pt 10): 1661-1670 (2000).
3. Yang A, Walker N, Bronson R, Kaghad M, Oosterwegel M, et al. Nature 404: 99-103 (2000).
4. Ueda Y, Hijikata M, Takagi S, Chiba T, and Shimotohno K. Oncogene 18: 4993-4998 (1999).
5. Sayan AE, BS Sayan, V Gogvadze, D Dinsdale, U Nyman, TM Hansen, B Zhivotovsky, GM Cohen, RA Knight, G Melino. p73 and caspase-cleaved p73 fragments localize to mitochondria and augment TRAIL-induced apoptosis. Oncogene 27:4363-4372 (2008).

Product Citations
1. Thioredoxin-Related Regulation of NO/NOS Activities. Li-en Shao, Tetsuya Tanaka, Ruby Gribi, and John Yu. Ann. N.Y. Acad. Sci., 962: 140-150 (2002). This antibody is referred to as mAb 789 in the publication. Western blot, Fig. 2B, Page 144.