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| Peptide-affinity Purified Polyclonal Antibody to ERp57/GRP58 |
| Catalog No | : | IMG-3145 | | Contents | : | 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. | | Isotype | : | Goat Ig | | Clone | : | N/A | | Purification | : | Antigen-Affinity Chromatography | | Species React | : | Human | | Predicted React | : | Hamster, Mouse, Rabbit, Rat | | Host | : | Goat | | | Application
Western blot analysis: 1-3ug/ml
ELISA: >1:8000
Storage
Store at -20°C.
Recommended Positive Control : A549 |
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Background
Principal Names – GRP58; glucose regulated protein, 58kDa; P58; ERP57; ERP60; ERP61; ERp57; ERp60; ERp61; GRP57; PI-PLC; glucose regulated protein, 58kD Official Gene Symbol - GRP58GenBank Accession Number – NP_005304 LocusLink ID - 2923 (human); 14827 (mouse); 29468 (rat)Gene Ontology terms - ER retention; phospholipase C; signal transduction; protein-nucleus import; cysteine-type endopeptidase; protein disulfide isomerase |
Antigen
Peptide with sequence EEKPKKKKKAQEDL, from C Terminus of the protein sequence according to NP_005304.
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Application Notes
Approx. 60 kDa band observed in A549 lysates (predicted MW of 58 kDa according to NP_005304). |
Genebank Info (Protein)
NP_005304
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 | Detection of ERp57 in A549 cell lysate (RIPA buffer, 35 µg total protein per lane) using IMG-3145 (1 µg/ml). Detected by western blot using chemiluminescence. | | | |
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Related Products
1. IMG-3073 [Peptide-affinity Purified Polyclonal Antibody to GAPDH - Loading Control]
2. 40165 [A549 cell line lysate (lung carcinoma)]
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Reference
1. Koivunen P, Horelli-Kuitunen N, Helaakoski T, Karvonen P, Jaakkola M, Palotie A, Kivirikko KI. Structures of the human gene for the protein disulfide isomerase-related polypeptide ERp60 and a processed gene and assignment of these genes to 15q15 and 1q21. Genomics. 1997 Jun 15;42(3):397-404. PMID: 9205111
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