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| Peptide-affinity Purified Polyclonal Antibody to Rabenosyn 5 |
| Catalog No | : | IMG-3288 | | Contents | : | 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. | | Isotype | : | Goat Ig | | Clone | : | N/A | | Purification | : | Antigen Affinity Chromatography | | Species React | : | Human | | Host | : | Goat | | | Application
Western blot analysis: 0.3-1.5 ug/ml
ELISA: >1:64000
Storage
Store at -20°C.
Recommended Positive Control : A431 |
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Background
Principal Names – Rabenosyn 5; FYVE-finger-containing Rab5 effector protein rabenosyn-5; zinc finger, FYVE domain containing 20; Official Gene Symbol - ZFYVE20 GenBank Accession Number – NP_071735 Gene ID - 64145 (human) |
Antigen
Peptide with sequence ELKHTLAKQKGGTD, from C Terminus of the protein sequence according to NP_071735
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Application Notes
An ~110 kDa band is observed in A431 lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). |
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 | IMG-3288 (0.3 µg/ml) staining of A431 lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. | | | |
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Related Products
1. IMG-3073 [Peptide-affinity Purified Polyclonal Antibody to GAPDH - Loading Control]
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Reference
1. Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M. Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain. J Cell Biol. 2000 Oct 30;151(3):601-12. PMID: 11062261
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