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Goat anti-Human LXR alpha / LXR beta - IMG-3380 |
| Imprimer |
| | | Peptide-affinity Purified Polyclonal Antibody to LXR alpha/LXR beta | | Catalog No | : | IMG-3380 | | Contents | : | 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. | | Isotype | : | Goat Ig | | Clone | : | N/A | | Purification | : | Antigen Affinity Chromatography | | Species React | : | Human | | Predicted React | : | Mouse, Rat | | Host | : | Goat | | | Application
Western blot analysis:1µg/ml
Storage
Store at -20°C.
Recommended Positive Control : Human Spleen lysate |
| Background
Principal Names – NR1H3; LXRA; LXR-a; RLD-1; nuclear receptor subfamily 1, group H, member 3; liver X receptor, alpha; NR1H2; NER; UNR; LXR-b; NER-I; RIP15; nuclear receptor subfamily 1, group H, member 2; ubiquitously-expressed nuclear receptor / NR1H2; NER; UNR; LXR-b; NER-I; RIP15; nuclear receptor subfamily 1, group H, member 2; ubiquitously-expressed nuclear receptor Official Gene Symbol - NR1H3; NR1H2GenBank Accession Number – NP_005684; NP_009052 Gene ID - 10062 (human); 7376 (human)Gene Ontology terms - nucleus; regulation of transcription, DNA-dependent; steroid hormone receptor activity; thyroid hormone receptor activity; transcription coactivator activity; transcription factor activityThis antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684) and to aa 443-456 of human LXR beta protein (NP_009052). |
Antigen
Peptide with sequence CRLQDKKLPPLLSEI, from Internal of the protein sequence according to NP_005684; NP_009052
| Application Notes
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55kDa band observed in Human Spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Additional minor bands were also consistently observed at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide. |
Genebank Info (Protein)
NP_005155
| |  | IMG-3380 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. | | | | | | Related Products
1. 40149 [Human Spleen Tissue lysate]
| | Reference
1. Tangirala RK, Bischoff ED, Joseph SB, Wagner BL, Walczak R, Laffitte BA, Daige CL, Thomas D, Heyman RA, Mangelsdorf DJ, Wang X, Lusis AJ, Tontonoz P, Schulman IG. Identification of macrophage liver X receptors as inhibitors of atherosclerosis. Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11896-901. Epub 2002 Aug 22. PMID: 12193651
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