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| Peptide-affinity Purified Polyclonal Antibody to Adenosine A2b Receptor |
| Catalog No | : | IMG-3883 | | Contents | : | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied as 100µg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. | | Isotype | : | Goat Ig | | Clone | : | N/A | | Species React | : | Human | | Host | : | Goat | | | Application
IHC (paraffin): 3µg/ml
Storage
Store at -20°C.
Recommended Positive Control : Human Kidney |
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Background
Principal Names- ADORA2B; adenosine A2b receptor; HGNC:264; ADORA2; netrin-1 receptor. Official Symbol- ADORA2B. GenBank Accession Number(s)- NP_000667.1. GeneID- 136 (human). |
Antigen
Peptide with sequence CQADVKSGNGQ, from the C Terminus of the protein sequence according to NP_000667.1.
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Application Notes
Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human Colon, Lung and Placenta lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules . Recommended concentration, 3µg/ml. |
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| | IHC analysis of Adenosine A2b Receptor in paraffin embedded Human Kidney using IMG-3883 at 3µg/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. | | | |
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Reference
1. Bazzichi L, Trincavelli L, Rossi A, De Feo F, Lucacchini A, Bombardieri S, Martini C. A2B adenosine receptor activity is reduced in neutrophils from patients with systemic sclerosis. Arthritis Res Ther. 2005;7(2):R189-95. Epub 2004 Dec 10. PMID: 15743465 [PubMed - in process
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