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| Affinity Purified Polyclonal Antibody to Caveolin-2 (Phospho-Ser36) |
| Catalog No | : | IMG-5014 | | Contents | : | 50 ug of purified IgG (0.5 mg/ml) with 50% glycerol, 0.01% thimerosal and 1.0 mg/ml BSA. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Protein G Chromatography | | Species React | : | Human | | Host | : | Rabbit | | | Application
Western blot analysis: 0.1-1 ug/ml
Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.
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Background
Caveolae are specialized plasma membrane associated organelles that participate in numerous cellular functions including endocytosis, potocytosis, transcytosis, signal transduction and cholesterol trafficking. The major proteins that form caveolae are the caveolins of which there are three major isoforms. Caveolin-2 has been shown to positively regulate caveolin-1-dependent caveolae biogenesis; this phenomenon is contingent on the phosphorylation of caveolin-2 on serine 23 and 36. |
Antigen
Rabbits were immunized with a synthetic peptide based on amino acid residues 32-45 of caveolin-2 phosphorylated on Ser36. Antibodies were purified by peptide affinity chromatography.
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Application Notes
An ~20 kDa band is observed. |
Genebank Info (Protein)
NP_058596
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 | Lysates from LNCaP cells expressing nonpohosphorylated and phosphorylated caveolin-2 were left untreated (-) or treated with (+) calf intestinal phosphatase (CIP). The phosphospecific caveolin-2 (Ser36) antibody (Cat No. IMG-5014) recognized the untreated, but not the CIP-treated lysate. | | | |
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Related Products
1. 20301 [Goat Anti-Rabbit HRP Conjugate]
2. IMG-5142A [Polyclonal Antibody to beta Actin]
3. IMG-5143A [Polyclonal Antibody to GAPDH]
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Reference
1. Sowa, G., M. Pypaert, D. Fulton, and W.C. Sessa. 2003. The phosphorylation of caveolin-2 and serines 23 and 36 modulates caveolin-1-dependent caveolae formation. PNAS 11:6511-6516. (Western blot)
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