Rabbit anti-Mouse / Human / Rat DARPP 32 (Thr75) - IMG-5042

Background
DARPP-32, a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.

Antigen
A synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr75 of DARPP-32 was used as immunogen. The antibody was purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns.

Application Notes
This polyclonal antibody is specific for the ~32 kDa DARPP-32 phosphorylated at Thr75. Researchers are encouraged to optimize dilutions for their specific testing.

Genebank Info (Protein)
NP_115568

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Reference
1. Svenningsson P.,  E.T. Tzavara, R. Carruthers, I. Rachleff, S. Wattler, M. Nehls, D.L. McKinzie, A.A. Fienberg, G.G. Nomiko and P. Greengard. 2003. Diverse psychotomimetics act through a common signaling pathway. Science 302:1412-1415.
2. Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G. Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London) 418 (2002) 774 - 778.
3. Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A. and Morrisett, R.A. “DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens” Nature Neurosci. 5 (2002) 641 - 648.
4. Bibb, J.A., Snyder, G.L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A.A., Tsai, L.H., Kwon, Y.T., Girault, J.A., Czernik, A.J., Huganir, R.L., Hemmings, Jr., H.C., Nairn, A.C. and Greengard, P. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signallingin neurons, Nature (London) 402 (1999) 669 - 671.