| Catalog No | : | IMG-5051 | | Contents | : | 0.1 ml antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western Blots. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Antigen Affinity Chromatography | | Species React | : | Rat | | Host | : | Rabbit | | | Application
Western blot analysis: 1:1000-1:2000
IF/ICC: 1:1000-1:2000
IHC (frozen): 1:1000
Storage
For long-term storage –80°C is recommended, but short-term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol.
Recommended Positive Control : KCl stimulated or unstimulated PC-12 (10 min, 30 mM) |
Background
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies are useful markers for dopaminergic and noradrenergic neurons. Short-term regulation TH is accomplished by dynamic changes in the phosphorylation state of the enzyme. Four phosphorylation sites in TH have been identified (Ser8, Ser19, Ser31, and Ser40), all in the amino-terminal regulatory region. Phosphorylation of tyrosine hydroxylase Ser19 has been shown to increase both Ser40 phosphorylation and enzyme activity of tyrosine hydroxylase in intact cells. |
Reference
1. Witkovsky P, Gabriel R, Haycock, J W, and Meller E. 2002. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J. Chem. Neuroanat., 19:105 - 116.
2. Haycock JW, Lew J Y, Garcia-Espana A, Lee, K Y, Harada K, Meller E, and Goldstein M. 1998. Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis. 1998. J. Neurochem., 71:1670 – 1675.
3. Xu Z.-Q, Lew JY, Harada K, Aman K, Goldstein M, Deutch A, Haycock J.W, and Hokfelt T. 1998. Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies. Neuroscience 82:727 – 738.
4. Bobrovskaya L, Dunkley PR, and Dickson PW. 2004. Phosphorylation of Ser19 increases both Ser40 phosphorylation and enzyme activity of tyrosine hydroxylase in intact cells. J Neurochem. 4:857-864
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