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Hamster anti-Mouse CD3, PE (Clone : 145-2C11) - IMG-5923D |
| Imprimer |
| | | Monoclonal Antibody to mouse CD3 (Clone 145-2C11) PE Conjugate | | Catalog No | : | IMG-5923D | | Contents | : | 100 ug in 200 ul PBS containing 0.05% sodium azide. Sodium azide is highly toxic. | | Isotype | : | Hamster IgG | | Clone | : | 145-2C11 | | Purification | : | Protein G Chromatography | | Species React | : | Mouse | | Host | : | Armenian Hamster | | | Application
Flow (Cell Surface): 0.1-1 ug/10^6 cells
Storage
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE.
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| Background
CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID. |
Antigen
H-2Kb specific cytotoxic T lymphocyte clone BM10-37
| | |  | Cell surface flow analysis of CD3 in BALB/c mouse splenocytes. Cells were stained with anti-CD19 APC (IMG-6294G) and either 1 ug of hamster isotype control antibody (left) or 1 ug of anti-CD3 PE (right, IMG-5923D). | | | | | | | | | | |
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