|
|
|
| Our products |
|
|
 |
|
|
|
|
|
|
|
|
| Other information |
|
|
|
Mouse anti-Human TIM-3, Azide-Free (Clone : F38-2E2) - IMG-6543E-50 |
| Imprimer |
| | | Monoclonal Antibody to human TIM-3 (Clone F38-2E2) Azide Free | | Catalog No | : | IMG-6543E-50 | | Contents | : | 50 ug in 100 ul PBS. Filter sterilized. Sodium azide free. Endotoxin level < 0.06 EU/ug. | | Isotype | : | Mouse IgG1, Kappa | | Clone | : | F38-2E2 | | Purification | : | Protein G Chromatography | | Species React | : | Human | | Host | : | Mouse | | | Application
Flow (Cell Surface): 0.5-1 ug/10^6 cells
Storage
Aliquot and store frozen at -20 degrees C.
|
| Background
TIM-3 has been identified as a Th1 specific marker initially due to expression on activated and polarized Th1 cells and absence on naive T cells. It has also been found on CD8+ T cells, Th17, Tregs , monocytes and dendritic cells. As a transmemebrane protein, TIM-3 is also referred to as 'T cell immunoglobulin and mucin-like domain containing protein -3.' Enhanced surface expression on CD4+ and CD8+ cells in the CNS are indicative of disease in EAE, murine models of MS. TIM-3 mediated down regulation of Th1 immune responses and induction of immune tolerance illustrate a potential key role for TIM-3 in immunoregulation. |
Antigen
Recombinant human TIM-3 protein was used as immunogen.
| | |  | Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with 0.6 ug of purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green) (IMGENEX, 20109).� IMGENEX's cell surface flow kit (10084K) and PE-conjugated secondary antibody (20103) was used for this test (cells were not fixed for testing). | | | | | | | Reference
1. Hastings, W.D. et.al. 2009 Eur. J. Immunol. 39: 2492
2. Hafler,D.A and V. Kuchroo, et.al. 2008 J. Exp. Med. 205:2699
3. Zhu, C et.al 2005 Nat. Immunol 6:1245
4. Su, E.W. Lin, V, and L.P. Kane 2008 Cytokine 44: 9
5. Kuchroo, V.K. et.al. 2003 Nat. Rev. Immunol.3:454
6. Monney, L. et al. 2002. Nature 415: 536
| | | | |
| |
