|Catalog No ||: ||IMG-671 |
|Contents ||: ||0.1 ml in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots. |
|Isotype ||: ||Rabbit Ig |
|Clone ||: ||N/A |
|Purification ||: ||Antigen Affinity Chromatography |
|Species React ||: ||Human, Mouse, Rat |
|Host ||: ||Rabbit || ||Application |
Western blot analysis: 1:1000
For long-term storage –80°C is recommended, but short-term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol.
Recommended Positive Control : Purified Recombinant Elk (Cell Signalling)
1. Hill, C.S. and Treisman, R. “Transcriptional regulation by extracellular signals: mechanisms and specificity,” Cell 80 (1995), 199 - 211.
2. Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D., and Martins-Green, M. “MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300.” EMBO J. 22 (2003), 281 - 91.
3. Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., “Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1,” J. Neurosci. 22 (2002) 2054 - 2062.