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| Peptide-affinity Purified Polyclonal Antibody to HSP27 (Ser15) |
| Catalog No | : | IMG-90257-1 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse, Rat | | Host | : | Rabbit | | | Application
Western Blot: 1:500-1:1000
IHC (paraffin): 1:50-1:100
Storage
Store at -20°C.
Recommended Positive Control : HeLa cells |
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Background
Human Hsp27 belongs to the phylogenically conserved small heat shock protein (smHsp) family, which includes mouse Hsp25, alphaA-crystallins and alphaB-crystallins. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. It is believed to exist mainly as oligomers of as many as 8-40 of Hsp27 protein monomers in cells and data suggest that these large oligomers have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is phosphorylated on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPKAP kinase 2/3 and the stress-activated protein kinase SAPK2 (p38). |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human HSP27 around the phosphorylation site of serine15 (G-P-SP-W-D).
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Genebank Info (Protein)
NP_001531
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 | Western blot analysis of HSP27 (Ser15) IMG-90257-1 in HeLa cells. | | | |  | Immunohistochemical analysis of HSP27 (Ser15) in paraffin-embedded human breast carcinoma tissue using IMG-90257-1. | | | |
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Reference
1. Mehlen, P. et al. Biochem. Biophys. Res. Commun. 241:187-192 (1997).
2. Benndorf, R. et al. J. Biol. Chem. 269:20780-20784 (1994).
3. Butt, E. et al. J. Biol. Chem. 276:7108-7113 (2001).
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