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| Peptide-affinity Purified Polyclonal Antibody to MEK1 (Ser217) |
| Catalog No | : | IMG-90282-1 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse, Rat | | Host | : | Rabbit | | | Application
IHC (paraffin): 1:50-1:100
Storage
Store at -20°C.
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Background
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK1 by MAP kinase. The sites on MEK1 that are phosphorylated by MAP kinase are Thr292 and Thr386. Human MEK1 gene is localized on chromosomal region 15q22.1-q22.33. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to human MEK1 around the phosphorylation site of serine 217 (I-D-SP-M-A).
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Genebank Info (Protein)
NP_002746
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|  | Immunohistochemical analysis of MEK1 (Ser217) in paraffin-embedded human breast carcinoma tissue using IMG-90282-1. | | | |
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Reference
1. Park, SH. et al. Science. 299:1061-1064 (2003).
2. Mansour, SJ. et al. J. Biochem. 116:304-314 (1994).
3. Crews, CM. et al. Science. 258:478-480 (1998).
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