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| Peptide-affinity Purified Polyclonal Antibody to VEGFR2/Flk-1/KDR (Ab-1175) |
| Catalog No | : | IMG-90419-2 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse, Rat | | Host | : | Rabbit | | | Application
IHC (paraffin): 1:50-1:100
Storage
Store at -20°C.
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Background
VEGFR2 is the major VEGF receptor in human malignancies. VEGFR2 is highly expressed in colon cancer endothelium and relates to increased tumour vascularity and high metastatic ability. Binding of VEGF to VEGFR2 on endothelial cells results in receptor phosphorylation and triggering of a cascade of events leading to endothelial cell proliferation, migration, apoptosis inhibition and maturation of vascular structures. Human VEGFR2 gene is mapped to chromosome 4q11-q12. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human VEGFR2 around the phosphorylation site of tyrosine 1175 (K-D-YP-I-V).
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Genebank Info (Protein)
NP_002244
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|  | Immunohistochemical analysis of VEGFR2 (Ab-1175) in paraffin-embedded human breast carcinoma tissue using IMG-90419-2. | | | |
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Reference
1. Chlouverakis, E. et al. European Journal of Clinical Investigation. 37:878-886 (2007).
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