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| Peptide-affinity Purified Polyclonal Antibody to PKD/PKCµ (Ab-738) |
| Catalog No | : | IMG-90456-2 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse, Rat | | Host | : | Rabbit | | | Application
Western Blot: 1:500-1:1000
Storage
Store at -20°C.
Recommended Positive Control : A431 cells |
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Background
PKD is a member of the protein kinase C (PKC) family functioning in many extracellular receptor-mediated signal transduction pathways. PKD differs from PKC isozymes by an acidic domain, a pleckstrin homology domain within the regulatory region and the lack of a typical pseudosubstrate site. PKD is responsible for the triggering of Golgi-mediated transport processes and for the maintenance of Golgi stacks architecture. PKD also participates in the control of keratinocyte proliferation to counteract TNF-induced apoptosis in HeLa cells. PKD is also involved breast cancer by triggering cellular adhesion processes. Human PKD gene is mapped to chromosomal region 14q11. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human PKD/PKCµ around the phosphorylation site of serine 738 (E-K-SP-F-R).
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Genebank Info (Protein)
NP_002733
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 | Western blot analysis of PKD/PKCµ (Ab-738) (Lanes 1 and 2) using IMG-90456-2 in A431 cells, untreated or treated with EGF (200ng/ml, 10min). | | | |
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Reference
1. Anna, T. et al. Oncogene. 22:8939–8947 (2003).
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