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Rabbit anti-Human / Mouse / Rat PKCq (Ab-695) - IMG-90499-2 |
| Imprimer |
| | | Peptide-affinity Purified Polyclonal Antibody to PKCq (Ab-695) | | Catalog No | : | IMG-90499-2 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse, Rat | | Host | : | Rabbit | | | Application
Western Blot: 1:500-1:1000
IHC (paraffin): 1:50-1:100
Storage
Store at -20°C.
Recommended Positive Control : HT-29 and K562 cells |
| Background
PKCq is a member of the novel, Ca(2+)-independent PKC subfamily, which plays an important and non-redundant role in several aspects of T cell biology. Mutation of PKCq gene leads to impaired receptor-induced stimulation of the transcription factors AP-1, NF-kappaB and NFAT, which results in defective T cell activation, and to aberrant expression of apoptosis-related proteins, resulting in poor T cell survival. It is the major isoform of PKC that is involved in NF-kB activation induced by CD3-CD28 costimulation. PKCq is expressed mostly in skeletal muscle, megakaryoblastic cells and platelets. Human PKCq gene is localized on human chromosome 10p15. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human PKCq around the phosphorylation site of serine 695 (N-F-SP-F-M).
| |  | Western blot analysis of PKCq (Ab-695) using IMG-90499-2 in HT-29 (Lane 1 and 2) and K562 cells (Lane 3) treated with Anisomycin (1mM, 30min). | | | |  | Immunohistochemical analysis of PKCq (Ab-695) in paraffin-embedded human lung carcinoma tissue using IMG-90499-2. | | | | | | | Reference
1. Xin, L. et al. Mol. Cell. Biol. 20:2933-2940 (2000).
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