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| Peptide-affinity Purified Polyclonal Antibody to Cortactin (Ab-466) |
| Catalog No | : | IMG-90561-2 | | Contents | : | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Isotype | : | Rabbit IgG | | Clone | : | N/A | | Purification | : | Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns. | | Species React | : | Human, Mouse | | Host | : | Rabbit | | | Application
Western Blot: 1:500-1:1000
IHC (paraffin): 1:50-1:100
Storage
Store at -20°C.
Recommended Positive Control : Hela cells |
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Background
Cortactin is a c-src substrate associated with sites of dynamic actin assembly at the leading edge of migrating cells promotes branched actin filament network formation by Arp2/3 complex. Cortactin is an F-actin-binding protein. The amino-terminal half of the protein is comprised of multiple copies of an internal, tandem 37- amino acid repeat while the carboxyl-terminal half contains a distal SH3 domain. Cortactin, present in several different cell types, is enriched in cortical structures such as membrane ruffles and lamellipodia. The properties of cortactin indicate its importance for microfilament-membrane interactions as well as transducing signals from the cell surface to the cytoskeleton. Human cortactin gene is localized on chromosome 11q13. |
Antigen
This antibody was generated by immunizing rabbits with a synthetic non-phosphopeptide corresponding to human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).
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Genebank Info (Protein)
NP_031829
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 | Western blot analysis of Cortactin (Ab-466) (Lanes 1 and 2) using IMG-90561-2 in Hela cells, untreated or treated with UV (20min). | | | |  | Immunohistochemical analysis of Cortactin (Ab-466) in paraffin-embedded human breast carcinoma tissue using IMG-90561-2. | | | |
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Reference
1. Wu, H. et al. The Journal of Cell Biology. 120:1417-1426 (1993).
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