Optimal dilution to be determined by the researcher.
Specificity and Use
PTBP1 antibody was raised against synthetic peptide DGIVPDIAVGTKR-C from the N-terminus of human PTBP1 (NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%); Horse, Zebrafish (85%).
N-Terminus
Human PTBP1. This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1; NP_114367.1; NP_114368.1; NP_787041.1).
Immunohistochemistry: LS-B3235 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3235 was determined to be 5 ug/ml.
Anti-PTBP1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3235 concentration 5 ug/ml.
Immunohistochemistry
Anti-PTBP1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3235 concentration 5 ug/ml.
Western Blot
Antibody staining (1 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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