Optimal dilution to be determined by the researcher.
Specificity and Use
PDIA4 / ERP72 antibody was raised against a 16 residue synthetic peptide (C)FIDEHATKRSRTKEEL based on the mouse ERp72 (residues 623-638) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat (100%); Hamster (94%); Human, Gorilla, Gibbon, Bovine, Turkey, Chicken (88%); Pig, Platypus (81%).
aa623-638
Detects an ~72 kD protein, corresponding to the apparent molecular mass of ERp72.
Immunohistochemistry: LS-B3756 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3756 was determined to be 10 ug/ml.
PDIA4 Antibody, ER protein 70 Antibody, ERp-72 Antibody, ERP70 Antibody, ERP72 Antibody, Protein disulfide-isomerase A4 Antibody, ER protein 72 Antibody
Anti-ERp72 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3756 concentration 10 ug/ml.
Immunohistochemistry
Anti-ERp72 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3756 concentration 10 ug/ml.
Immunohistochemistry
Anti-ERp72 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3756 concentration 10 ug/ml.
Immunohistochemistry
Anti-ERp72 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3756 concentration 10 ug/ml.
Western Blot
Western Blot Analysis of ERp72: Lane 1: MW Marker, Lane 2: Rat Liver Microsome Extract, Lane 3: Mouse Liver Microsome Extract, Lane 4: Human Liver Microsome Extract.
PDF Spec Sheet
Technical Help
