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Rabbit anti-Human COX-2 - LS-B410-50
Imprimer
Anti-COX-2 Antibody LS-B410 IHC-plus™ Rabbit anti-Human Polyclonal, C-Terminus, Unconjugated Details Applications Target: Human COX-2 Host: Rabbit Reactivity: Human, Mouse, Rat Clonality: Polyclonal Purification: Sterile Filtration Format: Unconjugated (Unconjugated)
IHC-P (1:500) Western blot (1:5000 - 1:10000) ELISA (1:10000 - 1:50000) Optimal dilution to be determined by the researcher.
Specificity and Use Immunogen: COX-2 antibody was raised against fusion protein from human PTGS2 / COX-2. Epitope: C-Terminus Specificity: GST fusion protein, the epitope of which maps to the carboxy-terminus of human Cox-2. Usage: Immunohistochemistry: LS-B410 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B410 was determined to be 1:500.
Packaging Presentation: 0.01% sodium azide. Storage: +4°C or -20°C, Avoid repeated freezing and thawing. Restrictions: For research use only.
1 Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells. Guan Z, Buckman SY, Miller BW, Springer LD, Morrison AR. The Journal of biological chemistry. 1998 273:28670-6.
[PubMed:9786861] 2 Induction of NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase expression by androgens in human prostate cancer cells. Tong M, Tai HH. Biochemical and biophysical research communications. 2000 276:77-81.
[PubMed:11006085] About COX-2 Uniprot: P35354 NCBI: NM_000963 NP_000954.1 Synonyms: PTGS2 Antibody, COX-2 Antibody, Cyclooxygenase-2 Antibody, COX2 Antibody, Cyclooxygenase 2b Antibody, HCox-2 Antibody, GRIPGHS Antibody, PGH synthase 2 Antibody, PGHS-2 Antibody, PHS II Antibody, PHS-2 Antibody, Prostaglandin H2 synthase 2 Antibody, PGG/HS Antibody, Prostaglandin G/H synthase 2 Antibody
Description: Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase; PGH synthase; Cox-2) is a key enzyme in prostaglandin biosynthesis.
Related Targets and Antibodies COX-2 Antibodies (54) Target Host Clonality Format Epitope Reactivity Applications COX-2 Mouse Monoclonal Human IHC-Fr, IHC-P COX-2 Antibody [COX 229] LS-B3296 Mouse Monoclonal (2) Applications: IHC-Fr, IHC-PEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-P, IP, WB COX-2 Antibody LS-B5270 Rabbit Polyclonal (1) Applications: IHC-P, WB, IPEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC-P, IP, WB COX-2 Antibody LS-B2145 Rabbit Polyclonal (1) Applications: IHC-P, WB, IP...Epitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC-P, WB COX-2 Antibody LS-B410 Rabbit Polyclonal (3) Applications: IHC-P, WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Goat Polyclonal Human, Mouse, Rat, Sheep ICC, IHC-P, WB COX-2 Antibody LS-B1608 Goat Polyclonal (2) Applications: IHC-P, ICC, WBEpitope: aa578-596 Reactivity: Hu, Mu, Rt ...Format: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-P, IP, WB COX-2 Antibody LS-B5206 Rabbit Polyclonal (1) Applications: IHC-P, WB, IPEpitope: Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-Fr, IHC-P, IP, WB COX-2 Antibody LS-C79822 Rabbit Polyclonal Applications: IHC-Fr, IHC-P, WB...Epitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat, Sheep IHC-P, WB COX-2 Antibody LS-C150711 Rabbit Polyclonal (1) Applications: IHC-P, WBEpitope: Reactivity: Hu, Mu, Rt ...Format: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat Flo, IF, WB COX-2 Antibody LS-C150699 Rabbit Polyclonal Applications: IF, WB, FloEpitope: Reactivity: Hu, Mu, RtFormat: FITC
COX-2 Rabbit Polyclonal Human ELISA, IF, IHC-P, WB COX-2 Antibody LS-C118419 Rabbit Polyclonal (3) Applications: IHC-P, IF, WB...Epitope: C-Terminus Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human IF, IHC, WB COX-2 Antibody LS-C162280 Rabbit Polyclonal (3) Applications: IHC, IF, WBEpitope: aa370-399 Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human IF, IHC, WB COX-2 Antibody LS-C175976 Rabbit Polyclonal (2) Applications: IHC, IF, WBEpitope: Thr594 Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-P, WB COX-2 Antibody LS-C171720 Rabbit Polyclonal (2) Applications: IHC-P, WBEpitope: N-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human IHC, WB COX-2 Antibody LS-C162279 Rabbit Polyclonal (2) Applications: IHC, WBEpitope: aa177-204 Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Mouse ICC, IHC-P, WB COX-2 Antibody LS-C150715 Rabbit Polyclonal (1) Applications: IHC-P, ICC, WBEpitope: Reactivity: MuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-P, WB COX-2 Antibody LS-C171721 Rabbit Polyclonal Applications: IHC-P, WBEpitope: N-Terminus Reactivity: Hu, Mu, RtFormat: Magnetic beads
COX-2 Rabbit Polyclonal Human, Mouse Flo, IHC-P, WB COX-2 Antibody LS-C121172 Rabbit Polyclonal Applications: IHC-P, WB, FloEpitope: Reactivity: Hu, MuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC, WB COX-2 Antibody LS-C123333 Rabbit Polyclonal Applications: IHC, WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC, WB COX-2 Antibody LS-C123334 Rabbit Polyclonal Applications: IHC, WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, IHC, WB COX-2 Antibody LS-C123335 Rabbit Polyclonal Applications: IHC, WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Mouse Monoclonal Human ELISA, IHC-P, WB COX-2 Antibody LS-C22398 Mouse Monoclonal Applications: IHC-P, WB, ELISAEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Human, Monkey, Sheep ICC, IHC, WB COX-2 Antibody LS-C22386 Mouse Monoclonal Applications: IHC, ICC, WBEpitope: aa580-599 Reactivity: Hu, Mo, SpFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IHC-P, WB COX-2 Antibody LS-C121670 Rabbit Polyclonal Applications: IHC-P, WBEpitope: N-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Monoclonal Human, Mouse ICC, WB COX-2 Antibody [EP1978Y] LS-C50111 Rabbit Monoclonal (1) Applications: ICC, WBEpitope: Reactivity: Hu, MuFormat: Unconjugated
COX-2 Goat Polyclonal Human ELISA, WB COX-2 Antibody LS-C20148 Goat Polyclonal (1) Applications: WB, ELISAEpitope: C-Terminus Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB COX-2 Antibody LS-C136936 Rabbit Polyclonal (1) Applications: WB, ELISAEpitope: Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Sheep WB COX-2 Antibody LS-C150706 Rabbit Polyclonal (1) Applications: WBEpitope: Reactivity: Hu, Mu, SpFormat: Unconjugated
COX-2 Rabbit Monoclonal Human WB COX-2 Antibody [EPR468(2)] LS-C152249 Rabbit Monoclonal (1) Applications: WBEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Human ELISA, Flo COX-2 Antibody LS-C26449 Mouse Monoclonal Applications: Flo, ELISAEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Rat ELISA, WB COX-2 Antibody [M9909232] LS-C84316 Mouse Monoclonal Applications: WB, ELISAEpitope: Reactivity: RtFormat: Unconjugated
COX-2 Rabbit Polyclonal Bovine, Horse, Human, Sheep WB COX-2 Antibody LS-C48347 Rabbit Polyclonal Applications: WBEpitope: C-Terminus Reactivity: Bo, Ho, Hu ...Format: Unconjugated
COX-2 Mouse Monoclonal Human Flo COX-2 Antibody LS-C26451 Mouse Monoclonal Applications: FloEpitope: Reactivity: HuFormat: FITC
COX-2 Mouse Monoclonal Rat ELISA COX-2 Antibody LS-C26452 Mouse Monoclonal Applications: ELISAEpitope: Reactivity: RtFormat: Unconjugated
COX-2 Mouse Monoclonal Bovine, Dog, Human, Sheep Flo COX-2 Antibody [CX229] LS-C150709 Mouse Monoclonal Applications: FloEpitope: Reactivity: Bo, Do, Hu ...Format: FITC
COX-2 Rabbit Polyclonal Human WB COX-2 Antibody LS-C137463 Rabbit Polyclonal Applications: WBEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat IP COX-2 Antibody LS-C171722 Rabbit Polyclonal Applications: IPEpitope: N-Terminus Reactivity: Hu, Mu, RtFormat: Sepharose
COX-2 Mouse Monoclonal Human COX-2 Antibody [4H12] LS-C95591 Mouse Monoclonal Applications: Epitope: N-Terminus Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Mouse, Rat COX-2 Antibody LS-C95941 Rabbit Polyclonal Applications: Epitope: C-Terminus Reactivity: Mu, RtFormat: Unconjugated
COX-2 Mouse Monoclonal Human ELISA, Flo, WB COX-2 Antibody LS-C79824 Mouse Monoclonal Applications: WB, Flo, ELISAEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Human ELISA, Flo, WB COX-2 Antibody LS-C22378 Mouse Monoclonal Applications: WB, Flo, ELISAEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Human ELISA, WB COX-2 Antibody LS-C22379 Mouse Monoclonal Applications: WB, ELISAEpitope: Reactivity: HuFormat: Unconjugated
COX-2 Rabbit Polyclonal Bovine, Human, Mouse, Sheep ELISA, WB COX-2 Antibody LS-C22383 Rabbit Polyclonal Applications: WB, ELISAEpitope: Reactivity: Bo, Hu, Mu ...Format: Unconjugated
COX-2 Rabbit Polyclonal Bovine, Human, Mouse, Sheep ELISA, WB COX-2 Antibody LS-C22394 Rabbit Polyclonal Applications: WB, ELISAEpitope: C-Terminus Reactivity: Bo, Hu, Mu ...Format: Unconjugated
COX-2 Goat Polyclonal Human, Mouse ELISA, WB COX-2 Antibody LS-C36001 Goat Polyclonal Applications: WB, ELISAEpitope: aa18-604 Reactivity: Hu, MuFormat: Unconjugated
COX-2 Rabbit Polyclonal Bovine, Human, Mouse, Sheep ELISA, WB COX-2 Antibody LS-C36002 Rabbit Polyclonal Applications: WB, ELISAEpitope: C-Terminus Reactivity: Bo, Hu, Mu ...Format: Unconjugated
COX-2 Mouse Monoclonal Human, Mouse ELISA, WB COX-2 Antibody LS-C22399 Mouse Monoclonal Applications: WB, ELISAEpitope: Reactivity: Hu, MuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB COX-2 Antibody LS-C22400 Rabbit Polyclonal Applications: WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Mouse Monoclonal Human, Mouse ELISA, WB COX-2 Antibody LS-C22401 Mouse Monoclonal Applications: WB, ELISAEpitope: Reactivity: Hu, MuFormat: Unconjugated
COX-2 Mouse Monoclonal Human, Mouse ELISA, WB COX-2 Antibody LS-C79829 Mouse Monoclonal Applications: WB, ELISAEpitope: Intracellular Reactivity: Hu, MuFormat: Unconjugated
COX-2 Rabbit Polyclonal Human, Mouse, Rat ELISA, WB COX-2 Antibody LS-C79820 Rabbit Polyclonal Applications: WB, ELISAEpitope: C-Terminus Reactivity: Hu, Mu, RtFormat: Unconjugated
COX-2 Mouse Monoclonal Rat ELISA COX-2 Antibody LS-C79828 Mouse Monoclonal Applications: ELISAEpitope: Reactivity: RtFormat: Unconjugated
COX-2 Mouse Monoclonal Human WB COX-2 Antibody LS-C22396 Mouse Monoclonal Applications: WBEpitope: C-Terminus Reactivity: HuFormat: Unconjugated
COX-2 Mouse Monoclonal Human, Sheep Flo COX-2 Antibody LS-C22388 Mouse Monoclonal Applications: FloEpitope: aa580-599 Reactivity: Hu, SpFormat: FITC
COX-2 Mouse Monoclonal Human, Sheep Flo COX-2 Antibody LS-C22390 Mouse Monoclonal Applications: FloEpitope: aa580-599 Reactivity: Hu, SpFormat: PE
Immunohistochemistry Anti-COX-2 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B410 dilution 1:500.
Western Blot Anti-Cox2 Antibody - Western Blot. anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1beta induced WISH cell extract (lane 2). Detection occurs using a 1:10000 dilution of antibody followed by 1:10000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
Western Blot Anti-Cox2 is shown to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (lane 1) and IL-1b induced WISH cell extract (lane 2). Detection occurs using a 1:10,000 dilution of antibody.
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