Optimal dilution to be determined by the researcher.
Specificity and Use
NPFF2 / NPFFR2 antibody was raised against n terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset, Horse (81%).
Reacts with the N terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein.
Immunohistochemistry: LS-B673 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B673 was determined to be 5 ug/ml.
Contains sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
Neuropeptide FF activates ERK and NF kappa B signal pathways in differentiated SH-SY5Y cells. Sun YL, Zhang XY, He N, Sun T, Zhuang Y, Fang Q, Wang KR, Wang R. Peptides. 2012 38:110-7. (ICC, WB; Human) [PubMed:22981806]