Target:	Human NPFF2 / NPFFR2
Host:	Rabbit
Reactivity:	Human
Predicted Reactivity:	Gibbon, Gorilla
Clonality:	Polyclonal
Purification:	Immunoaffnity chromatography
Format:	Unconjugated (Unconjugated)

IHC-P (5 µg/ml)

Western blot (1:500 - 1:1000)

Optimal dilution to be determined by the researcher.

Specificity and Use

Immunogen:	NPFF2 / NPFFR2 antibody was raised against n terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset, Horse (81%).
Epitope:	N-Terminus
Specificity:	Reacts with the N terminal sequence MNEKWDTNSSENWHPI and the C terminal sequence ELVMEELKETTNSSEI of the human NPFF2 protein.
Usage:	Immunohistochemistry: LS-B673 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B673 was determined to be 5 ug/ml.

Packaging

Presentation:	Contains sodium azide
Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Restrictions:	For research use only.

Publications (1)

Neuropeptide FF activates ERK and NF kappa B signal pathways in differentiated SH-SY5Y cells. Sun YL, Zhang XY, He N, Sun T, Zhuang Y, Fang Q, Wang KR, Wang R. Peptides. 2012 38:110-7. (ICC, WB; Human) [PubMed:22981806]

About NPFF2 / NPFFR2

Uniprot: Q9Y5X5 NCBI: NM_004885 NP_004876.1

Synonyms: NPFFR2 Antibody, G protein-coupled receptor 74 Antibody, HLWAR77 Antibody, G-protein coupled receptor 74 Antibody, Neuropeptide FF receptor 2 Antibody, Neuropeptide FF 2 Antibody, NPFF2 Antibody, Neuropeptide FF 2 receptor Antibody, NPGPR Antibody, GPR74 Antibody, NPFF-R2 Antibody, NPFF2 receptor Antibody, NPGP receptor Antibody

Description: The Neuropeptide FF 2 Receptor (NPFF2/HLWAR77/NPGPR) is a Neuropeptide Receptor GPCR.

(More About NPFF2 / NPFFR2)

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Technical Help

NPFF2 / NPFFR2 Antibodies (8)

Target

Host

Clonality

Format

Epitope

Reactivity

Applications

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

IHC-P

NPFF2 / NPFFR2 Antibody LS-A3304

Rabbit Polyclonal

(2)

50 µg

Applications: IHC-P

Epitope: C-Terminus

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

IHC-P

NPFF2 / NPFFR2 Antibody LS-A3302

Rabbit Polyclonal

(1)

50 µg

Applications: IHC-P

Epitope: Extracellular Domain

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

IHC-P

NPFF2 / NPFFR2 Antibody LS-A463

Rabbit Polyclonal

(1)

50 µg

Applications: IHC-P

Epitope: N-Terminus

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

IHC-P, WB

NPFF2 / NPFFR2 Antibody LS-B673

Rabbit Polyclonal

(2)

100 µg

Applications: IHC-P, WB

Epitope: N-Terminus

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

ELISA, IHC, WB

NPFF2 / NPFFR2 Antibody LS-C40752

Rabbit Polyclonal

100 µg

Applications: IHC, WB, ELISA

Epitope:

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

NPFF2 / NPFFR2 Antibody LS-C135552

Rabbit Polyclonal

(1)

50 µg

Applications: WB

Epitope: C-Terminus

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

NPFF2 / NPFFR2 Antibody LS-C135553

Rabbit Polyclonal

(1)

50 µg

Applications: WB

Epitope: Internal

Reactivity: Hu

Format: Unconjugated

NPFF2 / NPFFR2

Rabbit

Polyclonal

Human

NPFF2 / NPFFR2 Antibody LS-C162960

Rabbit Polyclonal

(1)

100 µg

Applications: WB

Epitope: aa2-32

Reactivity: Hu

Format: Unconjugated

Immunohistochemistry

Anti-NPFFR2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B673 concentration 5 ug/ml.

Immunohistochemistry

Anti-NPFFR2 antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B673 concentration 5 ug/ml.

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