Optimal dilution to be determined by the researcher.
Specificity and Use
CNTFR antibody was raised against human CNTFR
This AN-D3 antibody has been tested by flow cytometric analysis of the IMR-32 cell line. This can be used at less than or equal to 1 \mug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Staining of the IMR-32 cell line with 0.5 ug of Biotin Mouse IgG2a, K isotype control (blue histogram) or 0.5 ug of Biotin anti-human CNTFR alpha (purple histogram) followed by Streptavidin-PE. Total viable cells were used for analysis.