Optimal dilution to be determined by the researcher.
Specificity and Use
EPHA3 antibody was raised against synthetic peptide derived from the C-terminal region of the human EphA3 receptor.
On Western Blot analysis, this antibody reacts specifically with recombinant human EphA3 receptor without cross-reaction with EphA4 receptor expressed in transfected HEK-293 cells. The antibody reactivity was confirmed by Western Blot and Immunoprecipitation analyses. Reactivity has been confirmed with human EphA3 receptor transfected HEK-293 cell lysate. The molecular weight for human EphA3 receptor is ~110kD on Western blots. Based on sequence homology, the antibody should also recognize the rat or mouse EphA3 receptor.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml, Band at ~110kD. Immunoprecipitation: 5-10 ug. Positive control: Human EphA3 receptor transfected HEK-293 cell lysate.
PBS, pH 7.4, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.