Optimal dilution to be determined by the researcher.
Specificity and Use
Myc Tag antibody was raised against a synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Mouse, Rat (100%); Orangutan, Monkey, Marmoset, Tamarin, Panda, Cat (90%); Horse, Pufferfish, Paramecium, Salpingoeca (80%).
C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc genes in human cancers, such as Hela whole cell lysates
Packaging
Lyophilized.
40 µl deionized water
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
20 ug Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) LS-C51564 and half of th IP was loaded on the gel. The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232)
Western Blot
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration. A: 0.2 ug/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml) B: 0.1 ug/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml) C: 0.05 ug/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml) The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Western Blot
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration. A: 0.2 ug/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml) B: 0.1 ug/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml) C: 0.05 ug/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml) The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Western Blot
Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing LS-C51564 posses excellent performance in WB assay with surprising antibody dilution. Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 ug/ml) in assay. The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG.
ICC
Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) LS-C51564
PDF Spec Sheet
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