Optimal dilution to be determined by the researcher.
Specificity and Use
anti-Goat Serum as well as purified and partially purified N-Acylmannosamine-1-Dehydrogenase [Pseudomonas]. Cross reactivity against N-Acylmannosamine-1-Dehydrogenase from other sources is unknown
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of N-Acylmannosamine-1- Dehydrogenase [Pseudomonas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti- Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
N-acylmanosamino-1-Dehydrogenase Polyclonal Antibody-Western blot. Goat anti-N-acylmanosamino-1-Dehydrogenase antibody (LS-C59439 lot 8181) was used to detect purified N-acylmanosamino-1-Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.