Optimal dilution to be determined by the researcher.
Specificity and Use
Pyranose Oxidase [E. coli].
anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyranose Oxidase [E. coli]. Cross reactivity against Pyranose Oxidase from other sources is unknown
Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Pyranose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Pyranose Oxidase Polyclonal Antibody-Western blot. Goat anti-Pyranose Oxidase antibody (LS-C59443 lot 8178) was used to detect pyranose oxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.