Optimal dilution to be determined by the researcher.
Specificity and Use
Pdi1p, yeast protein disulfide isomerase.
Recognizes yeast Pdi1p.
Suitable for use in Western Blot and Immunofluorescence. Western Blots of yeast protein extracts: 1:10000 (ECL detection) reveal a ~65kD band. Initial dilution range: 1:2500-1:5000. Immunofluorescence: 1:250-1:2500.
PBS, pH 7.4, 0.05% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.