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8-hydroxy-2-deoxy Guanosine (8-OHdG) EIA Kit - SKT-120-480 |
| Imprimer |
| Product Name | DNA Damage, 8-OH-dG StressXpress ® EIA Kit | | Catalog # | SKT-120-480 | | Size | 5x96 well | | | | Alternate Product Sizes | SKT-120-96 | | Type | EIA Kit | | Description | 8-OHdG detection | | Research Area | Oxidative Stress | | Alternative Names | 8-OH-dG, 8OHG, 80G, 8 hydroxyguanine, DNA Damage ELISA, 8OHdG ELISA | | Species Reactivity | N/A, 8OHdG | | Sample Type | Urine, cell culture, plasma and other sample matrices | | Sensitivity | 30pg/mL | | Assay Range | 100-30pg/mL | | Incubation Time | 18hours | | Storage Temp | -20 °C | | Shipping Temp | Blue Ice, 4 °C | | Research Background | 8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11). | | References | - Maxey K.M., Maddipati K.R., Birkmeier J. (1992) Interference in enzyme immunoassays. J Clin Immunoassay 15: 116-120.
- Pradelles P., Grassi J., Maclouf J. (1990) Enzyme immunoassays ofeicosanoids using acetylcholinesterase. Methods Enzymol 187: 24-34.
- Maclouf J., Grassi J., Pradelles P. (1987) Development of enzyme-immunoassay techniques for the measurement of eicosanoids.
- Lin H., et al. (2004) A high-throughput and sensitive methodologyfor the quantification of urinary 8-hydroxy-2’-deoxyguanosine:Measurement with gas chromatography-mass spectrometry after singlesolid-phase extraction. Biochem J 380: 541-548.
- Bogdanov M.B., et al. (1999) A carbon column-based liquidchromatography electrochemical approach to routine8-hydroxy-2’-deoxyguanosine measurements in urine and other biologicmatrices: A one-year evaluation of methods. Free Radic Biol Med27(5/6): 647-666.
- Lee J., et al. (2005) Carvedilol reduces plasma8-hydroxy-2’-deoxyguanosine in mild to moderate hypertension. A pilotstudy. Hypertension 45: 986-990.
- Leinonen, J., et al. (1997) New biomarker evidence of oxidative DNAdamage in patients with non-insulin-dependent diabetes mellitus. FEBSLett 417: 150-152.
- Endo K., et al. (2006) Probucol and atorvastatin decrease urinary8-hydroxy-2’-deoxyguanosine in patients with diabetes andhypercholesterolemia. Journal of Atherosclerosis and Thrombosis 13:68-75.
- Kuo H., et al. (2007) Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG)and genetic polymorphisms in breast cancer patients. Mutat Res 631:62-68.
- Shen J., et al. (2007) 8-hydroxy-2’-deoxyguanosine (8-OH-dG) as apotential survival biomarker in patients with nonsmall-cell lungcancer. Cancer 109: 574-580.
- Beckman K.B., Ames B.N. (1997) Oxidative decay of DNA. J Biol Chem 272: 19633-19636.
- Epe B., et al. (1996) DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res 24: 4105-4110.
- Spencer J.P.E., et al. (1995) DNA strand breakage and basemodification induced by hydrogen peroxide treatment of humanrespiratory tract epithelial cells. FEBS Lett 374: 233-236.
- Floyd R.A. (1990) Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J 4: 2587-2597.
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19. Ofek Bar-Ilan, Connie C. Chuang, Denise J. Schwahn, Sarah Yang, Sanjay Joshi, Joel A. Pedersen , Robert J. Hamers, Richard E. Peterson and Warren Heideman. TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations. Environ. Sci. and Tech. Published online 2013 March 13. doi: 10.1021/es304514r 20. Cristina Llorente Izquierdo, Rafael Mayoral, Juana MarÃa Flores, Pilar GarcÃa-Palencia, Carme Cucarella, Lisardo Boscá, Marta Casado, and Paloma MartÃn-Sanz. Transgenic Mice Expressing Cyclooxygenase-2 in Hepatocytes Reveal a Minor Contribution of This Enzyme to Chemical Hepatocarcinogenesis. Am J Pathol. 2011 March; 178(3): 1361–1373. doi: 10.1016/j.ajpath.2010.11.074
21. O Bar-Ilan, CC Chuang, DJ Schwahn, S Yang, S Joshi, et. al. TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations. Environ. Sci. Technol. Publication Date (Web): March 19, 2013. DOI: 10.1021/es304514r | | |
