8-hydroxy-2-deoxy Guanosine (8-OHdG) EIA Kit - SKT-120-480
|Product Name ||DNA Damage, 8-OH-dG StressXpress ® EIA Kit|
|Catalog # ||SKT-120-480|
|Size ||5x96 well|
|Alternate Product Sizes ||SKT-120-96|
|Type ||EIA Kit|
|Description ||8-OHdG detection|
|Research Area ||Oxidative Stress|
|Alternative Names ||8-OH-dG, 8OHG, 80G, 8 hydroxyguanine, DNA Damage ELISA, 8OHdG ELISA|
|Species Reactivity ||N/A, 8OHdG|
|Sample Type ||Urine, cell culture, plasma and other sample matrices|
|Assay Range ||100-30pg/mL|
|Incubation Time ||18hours|
|Storage Temp ||-20 °C|
|Shipping Temp ||Blue Ice, 4 °C|
|Research Background ||8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).|
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