Rabbit anti-Phosphoserine - SPC-149F
|Product Name ||Phosphoserine Antibody|
|Catalog # ||SPC-149F|
|Package size ||400ul|
|Datasheet ||SPC 149 Phosphoserine Cell Signaling|
|Research Area ||Cell Signaling, Phosphorylation, Post-translational Modifications|
|Alternative Names ||N/A|
|Clone Number ||N/A|
|Host Species ||Rabbit|
|Immunogen ||Phosphoserine conjugated to KLH, and phosvitin mixture|
|Applications ||WB, ELISA, IP, IHC|
|Species Reactivity ||Species Independent|
|Accession Number ||N/A|
|Background Info ||Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.|
|Recommended Dilutions ||2-4Î¼gmL (WB), 10 Î¼g/mg (IP), 0.5-4Î¼g/mL (ELISA)|
|Form ||Affinity Purified|
|Storage Buffer ||PBS, 50% glycerol, 0.09% sodium azide|
|Concentration ||250 µg/mL|
|Certificate of Analysis ||2μg/mL of SPC-149 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1μM okadaic acid.|
|Storage Temp ||-20 °C|
|Shipping Temp ||Blue Ice or 4 °C|
Western blot analysis of the phosphorylated proteins with UV-treated cell lysates mouse spleen cell. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1μM okadaic acid).
|Research Background ||Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).|
|References ||1. Goto H. et al. (2005). Nature Cell Biology 8: 180-187.|
2. Blume-Jensen P. and Hunter T. (2001). Nature 411: 355- 365.
3. Downward J. (2001). Nature 411: 759-762.
4. Pawson T. and Saxton T.M. (1999). Cell 97: 675-678.
5. Ostrovsky PC. (1995). Genes Dev. 9(16): 2034-2041.