Product Name	p38 Antibody
Catalog #	SPC-172D
Package size	100ul

Alternate Product Sizes	SPC-172C
Type	Polyclonal
Conjugate	N/A
Datasheet	SPC 172 p38 Cell Signaling
Description	Anti-p38
Research Area	Cell Signaling
Alternative Names	CSAID Binding protein 1, CSBP1, CSBP2, EXIP, MAP kinase MXI2, MAPkinase p38alpha, MAPK14, p38 ALPHA, p38 MAP kinase, p38 mitogen activated protein kinase, RK, SAPK 2A, Stress activated protein kinase 2A
Clone Number	N/A
Host Species	Rabbit
Isotype	N/A
Immunogen	A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH.
Applications	WB, IP, IHC
Species Reactivity	Human, Monkey, Mouse, Rat, Bovine, Rabbit, Pig, Canine, Hamster, Chicken, Sheep, Guinea pig
Accession Number	NP_001306.2
Gene ID	1433
SwissProt	Q16540
Background Info	Detects a ~43kDa protein corresponding to the molecular mass of p38 on SDS PAGE immunoblots.
Recommended Dilutions	1:5000 (ECL) (WB), 1:250 (IP)
Form	Rabbit Antiserum
Storage Buffer	Rabbit Antiserum
Concentration	N/A
Certificate of Analysis	A 1:5000 dilution of SPC-172 was sufficient for detection of p38 in 20μg of HeLa cell lysate by ECL immunoblot analysis.
Storage Temp	-20 °C
Shipping Temp	Blue Ice or 4 °C

Multi-blot analysis of p38 in a cell lysate from 12 human cancer cell lines using a 1:1000 dilution of SPC-172.

Research Background

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

References

1. Pearson, G. et al (2001). Endocrine Reviews 22 (2): 153-183.
2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38.
3. Han, J. et al. (1994) Science 265: 808-811.
4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948.
5. Deng et al. (2003) Cell. 115: 61-70.
6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899- 907.
7. Medicherla S. et al. (2006). J Pharmacol Exp Ther. 318(1): 99-107.