Gene Name	Homo sapiens methyl-CpG binding domain protein 1 (MBD1), transcript variant 4
Synonyms:	CXXC3; PCM1; RFT
Immunogen	A synthetic peptide corresponding to residues in human MBD1 was used as an immunogen
Buffer	Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Clone Name	EPR3564	Isotype	Rabbit IgG
Species Reactivity	Human	Concentration
Purification	(Protein A or G Sepharose )
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:20, WB: 1:500 - 1000,
Background	Methyl-CpG-binding domain protein 1 (MBD1) is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides (1). Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). MBD1 acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. It is also believed to form a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (2).
Related Pathway

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Western blot analysis on (A) HeLa (B) A431 (C) 293T and (D) Jurkat cell lysates using anti-MBD1 RabMAb .

Immunohistochemical analysis of paraffin-embedded human tonsils using anti-MBD1 RabMAb .

Immunofluorescent staining of MCF7 cells using anti-MBD1 RabMAb .

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Rabbit monoclonal antibody against MBD1(clone EPR3564) - TA307448

Anti-MBD1 Antibody EPR3564