Rabbit polyclonal antibody to RAG2 (recombination activating gene 2) - TA307957
Also for RAG2 (NM_000536)
|Homo sapiens recombination activating gene 2 (RAG2), transcript variant 1|
|Recombinant protein fragment contain a sequence corresponding to a region within amino acids 271 and 519 of RAG2|
|0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
| ||IgG |
|Human ||0.37mg/ml |
|Purified by antigen-affinity chromatography. (Protein A or G Sepharose ) |
|WB, IHC, IF ||WB:1:500-1:3000; IHC:1:100; IF:1:100-1:200; |
|This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]|
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Sample (30 ug of whole cell lysate) A: Molt-4 (anti-RAG2 antibody diluted at 1:1000)
Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(anti-RAG2 antibody) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(anti-RAG2 antibody) antibody at 1:200 dilution.