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Purified anti-BUB1B (BubR1) mouse monoclonal antibody, clone 3C7 - TA500534 |
| Imprimer |
 Anti-BUB1B TrueMAB Antibody clone 3C7 TrueMAB™ Antibodies - Made against Authentic Protein Antigens 
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.  Also for BUB1B (NM_001211) OriGene Data | Protein expressed in 293T cell transfected with human BUB1B expression vector | | clone 3C7 | IgG1 | | Human | 0.5~1.0 mg/ml (Lot Dependent) | | WB, IHC, IF | WB 1:2000 IHC 1:50 IF 1:100 | | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. | | Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose ) | Reference Data | Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) | | Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1 | | NP_001202 | | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. | Cell cycle | * Shipping is in business days * OriGene provides validated application data and protocol, with money back guarantee. WB Image HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B. IHC Image Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IHC Image Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (TA500534, Dilution 1:50) IF Image Anti-BUB1B mouse monoclonal antibody (TA500534) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844). Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules: - HRP
- Biotin
- PE
- FITC
- DyLight ® 488
- DyLight ® 550
- DyLight ® 594
- DyLight ® 633
* DyLight is a trademark of Thermo-Fisher Scientific Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation. Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support) |
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