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Purified anti-BUB1B (BubR1) mouse monoclonal antibody, clone 10B7 - TA500535

Imprimer

Anti-BUB1B TrueMAB Antibody clone 10B7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

Specifications

OriGene Data

Immunogen	Protein expressed in 293T cell transfected with human BUB1B expression vector
Clone Name	clone 10B7	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:2000 IHC 1:50 IF 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose )

Reference Data

Target Name	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B)
Alternative Name	Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1
Database Link	NP_001202
Function	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Related Pathway	Cell cycle

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (TA500535, Dilution 1:50)

IF Image

Anti-BUB1B mouse monoclonal antibody (TA500535) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).

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