Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201159 . Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 19.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families:

Protein Data

RefSeq: NP_003333	RefSeq Size: 4208	RefSeq ORF: 513
LocusID: 7326	Cytogenetic: 17p13.2
Synonyms : E217K; UBC7; UBE2G
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2G 1 (UBE2G1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2G 1 (UBE2G1) as transfection-ready DNA • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1) , Myc-DDK-tagged • Lenti ORF clone of Human ubiquitin-conjugating enzyme E2G 1 (UBE2G1), mGFP tagged Related Lysate from OriGene • Transient overexpression lysate of ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast) (UBE2G1) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • UBE2G1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • UBE2G1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ube2g1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • UBE2G1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ube2g1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene UBE2G1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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