Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202054 . Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 85.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Pathways:

Protein Data

RefSeq: NP_004447	RefSeq Size: 2695	RefSeq ORF: 2256
LocusID: 2146	Cytogenetic: 7q35
Synonyms : ENX-1; ENX1; EZH1; KMT6; KMT6A; WVS2
Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of enhancer of zeste homolog 2 (Drosophila) (EZH2), transcript variant 1 Validated Antibodies • Rabbit monoclonal antibody against Ezh 1/2 (clone EP1408Y ) • Rabbit monoclonal antibody against Ezh 2 Phospho (pT487)(clone EPR1410) (Phospho-Specific) • EZH2 mouse monoclonal antibody,clone 10B10 • Carrier-free (BSA/glycerol-free) EZH2 mouse monoclonal antibody,clone 10B10 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • EZH2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • EZH2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ezh2 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • EZH2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ezh2 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene EZH2

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity ( µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone