Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202718 . Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 20 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Protein Data

RefSeq: NP_001876	RefSeq Size: 691	RefSeq ORF: 528
LocusID: 1410	Cytogenetic: 11q22.3
Synonyms : CRYA2; CTPP2; HSPB5
Summary: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens crystallin, alpha B (CRYAB) as transfection-ready DNA • Lenti ORF clone of Human crystallin, alpha B (CRYAB) , Myc-DDK-tagged • Lenti ORF clone of Human crystallin, alpha B (CRYAB), mGFP tagged • GFP-tagged ORF clone of Homo sapiens crystallin, alpha B (CRYAB) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens crystallin, alpha B (CRYAB) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of crystallin, alpha B (CRYAB) Validated Antibodies • Rabbit Monoclonal Antibody against CRYAB (Clone EPR2751Y) • Rabbit Monoclonal Antibody against CRYAB (Clone EPR2752) • Purified Goat Polyclonal Antibody against CRYAB • Mouse Monoclonal anti-CRYAB Antibody • Rabbit Polyclonal anti-CRYAB Antibody • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 2A1 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 3C2 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 3E3 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 6A9 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 4A6 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 6D11 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 5C9 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 1G1 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 4G5 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone 6G5 • CRYAB (Alpha B Crystallin) mouse monoclonal antibody, Clone 5D8 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 3C2 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 3E3 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 6A9 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 4A6 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 6D11 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 5C9 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 1G1 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 4G5 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 6G5 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody, clone 2A1 • Carrier-free (BSA/glycerol-free) CRYAB mouse monoclonal antibody,clone 5D8 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CRYAB - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CRYAB - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cryab - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CRYAB - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cryab - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CRYAB

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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